Comparison of Polyphenol Levels of Callus and Wild Type of Cat's Whiskers Plant ( Orthosiphon aristatus (Blume) Miq.) Purple Varieties

The cat's whiskers plant ( Orthosiphon aristatus (Blume) Miq) that grows in Indonesia has white flowers, purple and flowers with patterns. quality of the secondary metabolite content each is different because it depends on environmental influences such as rainfall or soil mineral content. Plant tissue culture methods callus cell are used to overcome these obstacles do not depend environment can produce metabolites those produced by original plant. Cat's contain main compounds: rosmarinic acid, eupatorin, sinensetin. Rosmarinic acid belongs phenolic group, while sinensetin eupatorin flavonoids. This study aimed determine total levels polyphenols flavonoids cat ratio wild-type plants—determination polyphenol using Folin Ciocalteau reagent. Determining flavonoid based colorimetry AlCl 3 reagent, specific for flavone flavonol compounds. contained ethanolic extract variety were 16.056 ± 0.204 mgQE/g 30.780 0.425 mgQE/g, ethyl acetate leaves was 15,489 13,262 0.044 mgQE/g. ethanol leaf 1.39±0.03 0.16±0.02 2.16±0.012 2.24±0.010 more significant than wild type ) leaf.